Food and Fermentation Industries >

2010 , Vol. 36 >Issue 08: 83 - 88

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2010.08.032

Food and Fermentation Industries

Study on Hydrolysis of Mytilus coruscus Protein with Alcalase Protease

  • Dai Zhi-yuan ,
  • Zhang Ting ,
  • Zhang Yan-ping ,
  • Lu Yan-bin ,
  • Zhang Yan-ping
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Online published: 2010-08-25

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Abstract

Alcalase2.4L were applied to hydrolyze the Mytilus coruscus protein.Based on the results of single-factor tests,reducing power A700 was chosen as the response values.Using Response Surface Methodology(RSM),the optimum enzyme hydrolysis conditions of Alcalase2.4L to achieve the maximum antioxidant ability were as follows:substrate concentration([S]) 3.20%,[E]/[S]2.12%,enzymolysis temperature 55.54℃,and enzymolysis time 2 hours.Under such conditions,reducibility of the hydrolysate reached 0.587,which also had strong scavenging activity of DPPH radical and superoxide anion radical.

Key words： mytilus coruscus; enzymatic hydrolysis; antioxidation; response surface methodology

Cite this article

Dai Zhi-yuan , Zhang Ting , Zhang Yan-ping , Lu Yan-bin , Zhang Yan-ping . Study on Hydrolysis of Mytilus coruscus Protein with Alcalase Protease[J]. Food and Fermentation Industries, 2010 , 36(08) : 83 -88 . DOI: 10.13995/j.cnki.11-1802/ts.2010.08.032

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