Food and Fermentation Industries

Determination of Cordycepin and Uridine and Adenosine in Health Foods by High Performance Liquid Chromatography

  • Ding Xian-rong ,
  • Ti Gao-lan ,
  • Yang Lin ,
  • Wang Qing-qi ,
  • Jiang Tao
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Online published: 2010-09-25

Abstract

The cordycepin,uridine and adenosine in samples were extracted by water at 80oC,separated with CALESIL ODS-100 Cl8 column(5μm,4.6×250 mm),10mmoL/L monopotassium phosphate(pH=6.0),methanol(85∶15,V/V)in mobile phase,and detected at UV 260nm.The detection limit of the three components was 0.02μg/mL,0.01μg/mL and 0.01μg/mL(count by concentration of sample injection).The recovery rates were 96.7% and 96.4% and 99.4%.Relative standard deviation were 1.8% and 0.68% and 0.10%.The method is simple,quick,accurate and can be applied for detection of cordycepin and Uridine and adenosine in foods.

Cite this article

Ding Xian-rong , Ti Gao-lan , Yang Lin , Wang Qing-qi , Jiang Tao . Determination of Cordycepin and Uridine and Adenosine in Health Foods by High Performance Liquid Chromatography[J]. Food and Fermentation Industries, 2010 , 36(09) : 143 -147 . DOI: 10.13995/j.cnki.11-1802/ts.2010.09.021

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