A strain named Lactobacillus plantarum GB01-21 that can produce γ-aminobutyric(GABA) efficiently was screened in our laboratory.During the 20 hours,200 g/L L-Glu can be transformed into 140g/L GABA.In this work,γ-aminobutyric was separated and purified from the whole-cell transforming solution.The decolorizing technical conditions such as temperature,time,pH and amount of active carbon were studied.As a result,the optimum decolorization conditions were determined through single factor and orthogonal experiments as follows: activated carbon dosage 1.5%,decolorization temperature 70 ℃,pH value 4.0 and decolorization time 30 min.Under these conditions,the decolorization rate and recovery rate of bioconversion broth reached 98.42% and 97.23%,respectively.Finally,γ-aminobutyric acid was isolated and purified from bioconversion broth and the recovery rate and the purity of γ-aminobutyric acid was 89.4% and 96.7%,respectively.These findings pave the way for industrial production of GABA.