To establish a technical procedure for the purification of flavonoids from apple.Three types of macro porous resin(AB-8,DM-130,and NAK-9) were used to absorb and desorb apple flavonoid.The rate of absorption and desorption were compared among the resins.The conditions of absorbability and desorption were further investigated.The absorbability rate of three types of macro porous resin were 70.55%,62.45%,74.13% respectively,The desorption rate were 92.66%,86.84%.87.22% respectively.The results indicated that AB-8 macroporous resin was the best.The optimum adsorption conditions were as follows: sample concentration 64 mg/ mL,pH6.0 and the feeding rate 1 mL/ min.The optimum desorption parameters were as follows: ethanol concentration 70%,the flow rate.1.6 mL/min.The reclaimed ratio was 89.46% and the purity of the flavonoids extracted from apple fruit was 92.5%.
Wang Ying
,
Zhang Yu-gang
,
Dai Hong-yi
. Purification of Flavonoid from Apple with Macroporous Resin[J]. Food and Fermentation Industries, 2010
, 36(11)
: 166
-170
.
DOI: 10.13995/j.cnki.11-1802/ts.2010.11.012