The effect of Pulsed High Magnetic Field(PHMF) on milk peroxidase,lipase,catalase of milk was studied in this paper.And the results showed that: PHMF has great effect on the inactivation of enzymes mentioned above,and the order of the inactivation effect on the three enzymes was: Catalase> Lipase> milk peroxidase.The max inactivation rate of milk peroxidase,Lipase and Catalase is 47.5%,53.6% and 90.3% respectively under the condition o8.01T~18.69T magnetic field intensity,0~10 pulsed numbers.Regression equations about the enzyme inactivation effect were set up through the binary regression analysis method.The influencing factors of these models were magnetic field intensity and pulse count.The reliability of the models were tested.The results showed the following: the magnetic field intensity and pulse numbers have significant influence on the enzymes mentioned above,and more over,these factors have synergy effect on lipase.This paper provided a new method for milk pretreatment,and discussed the mechanism of PHMF on enzymes in milk.The research of this paper has important significance for the liquid food sterilization and enzyme inactivation.It also provides a theoretical basis in the related field of industrialized production.