This investigation focused on the utilization and ethanol fermentation of levoglucosan by the yeast Pichia stipitis.Results revealed that P.stipitis could utilize levoglucosan at a low efficiency compared to glucose,and could not ferment it to ethanol.However,levoglucosan could facilitate the growth of P.stipitis and enhance its ethanol production when both levoglucosan and glucose existed in culture media.In YPD media with 0.1% levoglucosan,the enhancement of cell concentration and ethanol production was approximately 14.3% and 25.4%,respectively.The results would support that levoglucosan could be used as an additive in high cell density fermentation with P.stipitis.