Wuxistatin was a novel inhibitor of 3-hydroxy-3-methylglutary(HMG)-CoA reductase which was the rate-limiting enzyme in the process of cholesterol synthesis.Amycolatopsis sp.ST2710 could convert lovastatin into wuxistatin.ProductⅠ,as the intermediate,was the key factor of producing wuxistatin.It was found that two products had different optimum pH.To increase the yield,the study took pH as the main factor to investigate by three ways;adjusting pH during fermentation;adjusting pH cooperated with adding fresh bacteria solution;separative subsection fermentation.Finally we found that the separative subsection fermentation had the best result.So the method was introduced;firstly,productⅠwas formed from lovastatin,pH 7.5 and pured by macroporous resin;then the conditions of fermentation were changed to pH 5.5 for transferring pured productⅠto wuxistatin.At the same time,the effect of some metal ions on transformation was studied and the result showed that Fe2+,Mg2+ and Cu2+ could improve fermentation level.By this way,the convertion would increase by 15%,the time was shorten by a big margin and the final product would be more easily to purify.The fermentation pattern that based on one strain could provide reference for the study of fermentation with intermediate product and long-time fermentation.