This study mainly investigated the protection of salt-induced soy protein isolate(SPI) gels on lactic acid bacteria in gastric acid.SPI was induced by CaCl2 to form the cold-set gel.The protection on lactic acid bacteria was determined by the survival rate of 30 minute in gastric acid.From the orthogonal experiment analysis,we can obtain the optimal experimental solution: A3B3C2.The solution show that the cold-set gel of 9% SPI induced at 85 ℃ by 20 mmol/L CaCl2 can give the best protection on lactic acid bacteria in the gastric acid condition of pH 1.2,and the order of lactic acid bacteria treated with the acid condition of pH 1.2 for 30 minute from 107 by the initial down to 102,dropped five orders.This suggested that this cold-set gels can provide better protection on lactic acid bacteria,and can be a prospect in the development and application of probiotics in food.