Shelf life of chilled chicken is associated with species and the numbers of microorganisms.Predominant spoilage bacteria in chilled chicken breast were isolated using selective media.The shelf life of chilled chicken breast at low storage temperatures were analyzed.The results indicated that Pseudomonas,Brochothrix thermosphacta,Enterobacteriaceae and Lactobacillus were the predominant spoilage bacteria in chilled chicken breast.The numbers of Pseudomonas and Brochothrix thermosphacta increased progressively and quickly became dominant microflora at low storage temperatures.The shelf life of chilled chicken breast was 14 d,10 d,5 d and 3 d at storage temperatures of 2℃,4℃,8℃ and 10℃ respectively.