Food and Fermentation Industries >

2011 , Vol. 37 >Issue 01: 184 - 188

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2011.01.042

Food and Fermentation Industries

Establishment of Aqueous Two-phase Extraction System for Ginger Protease

  • Xie Fang ,
  • Tang Xiao-zhen ,
  • Ma Ming ,
  • Du Xin-yong ,
  • Lu Hui-zhong
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Online published: 2011-01-25

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Abstract

To make use of ginger,extraction technology of ginger protease by Aqueous Two-phase System was studied using single factor and orthogonal experiments.Results indicated that the optimum PEG molecular weight was 4000,PEG concentration was 30%,sort of salts was(NH4)2SO4,(NH4)2SO4 concentration was 10% and pH of system was 8.0.Under the above extraction conditions,the GP activity recovery and purification factor of top phase in aqueous two-phase system reached maximums at 393.77% and 1.85.

Key words： aqueous two-phase system(ATPS); ginger protease(GP); ginger protease activity; ratio

Cite this article

Xie Fang , Tang Xiao-zhen , Ma Ming , Du Xin-yong , Lu Hui-zhong . Establishment of Aqueous Two-phase Extraction System for Ginger Protease[J]. Food and Fermentation Industries, 2011 , 37(01) : 184 -188 . DOI: 10.13995/j.cnki.11-1802/ts.2011.01.042

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