The liquid-liquid extraction process of modified lipid prepared by lipase catalyzed transesterification was investigated.The effects of extraction solution concentration,material ratio,extraction time on free fatty acid extraction ratio and modified lipid yield were investigated by single factor experiment and response surface methodology.The results show that free fatty acid extraction ratio is increasing with extraction solution concentration,material ratio,extraction time increasing,while yield is decreasing with these three process conditions increasing.The regression model from response surface methodology can highly reflect the relationship of different factor levels and free fatty acid extraction ratio,yield.The sequence of two factors influenced on free fatty acid extraction ratio of modified lipid is extraction solution concentration>material ratio,while the sequence of two factors influenced on yield of modified lipid is reversed,material ratio>extraction solution concentration.The optimal process is obtained by overlaying two contour lines of free fatty acid extraction ratio and modified lipid yield,and the optimal process is enthanol aqueous solution concentration 85.00%,material ratio 1∶ 1.