Kosteletzkya virginica seeds were taken as raw materials and the aqueous enzymatic extraction technology was used to extract seed oil.The single factor experiment and the response surface methodology were adopted to study the effects of solid to water ratio,extraction temperature,the ratio of enzyme to seeds,extraction time and pH on oil extraction rate.The order of these factors in terms of the influence on the oil extraction rate within the experimental range was as follows: the ratio of enzyme to seeds>extraction temperature >the ratio of solid to water>extraction time.The optimal technological parameters of oil extraction from Kosteletzkya virginica seeds should be as follows: the ratio of enzyme to seeds 0.024 mL/g,extraction temperature 63℃,the ratio of solid to water 1/6 and extraction time 230 min.Under such conditions,total oil extraction rate is 24.281%.