A new protocol to analyze tetracyclines and sulfonamides from pig muscle was developed.Six antibiotics,oxytetracycline,tetracycline,chlortetracycline,slfamonomethoxine,sulfamethazine,and sulfamerazine,were analyzed by microbial assays and HPLC.Muscle juice samples were screened with Bacillus sterothermophilus in 96-well microplate.At the same time,the muscles samples were extracted with Mcllvaine-EDTA buffer solutions and then were detected at 270nm and 360nm.The detection limits of microbial assay were 0.02~0.05μg/mL.For HPLC method,the limits of detection were 0.03~0.06 mg/kg,and the average recoveries were 80.25%~118.60%.