The characteristics of product inhibition on the growth of Actinobacillus succinogenes in fermentation using glucose as the major carbon source and the technology of the In Situ Product Removal(ISPR) with resin adsorption to alleviate the product inhibition were studied.The study showed that the characteristic of the cells growing process and the succinic acid synthesizing process was non-coupling,which means that the cells started to synthesize succinic acid in the stationary growth stage(after cultivation for 30 hours).The product inhibition became more and more obvious when the succinic acid concentration was over 25g/L in the fermentation broth,and the conversion rate of the substrate became decreased.Through simulative fermentation broth,resin of D301T,D301R and D303 have been selected,which have great adsorptive capacity of the succinic acid.D301R was innocuous in the ISPR,which was finally ascertained by using in the real fermentation system.Application effect of the ISPR with resin was confirmed through 5L automatic fermentor,and 40 g/L D301R resin was added after 30 hours cultivation.After the fermentation process was finished,the concentration of the succinic acid can reach 49.46g/L(the substrate of glucose is 60 g/L) and the glucose conversion rate can reach 82.43%,the substrate conversion rate was increased by 21% comparing with usual fermentation Method.