Fermented grass carp inoculated with four different starter cultures(Pediococcus acidilactici,Lactobacillus-Lo3,Lactobacillus-B5407 and Lactobacillus sake) were evaluated for physicochemical properties and proteolytic changes in this work.The result showed that grass carp muscle inoculated with starter cultures resulted in a rapid pH decrease from 6.25 to 5.06~5.40,but the control group was 5.98 during the 2 days fermentation.The TBA and TVB-N values was significantly lower than the contrl group(P<0.05) and rising slowly.Moreover,a few slight changes in proteolytic and increase in free amino acids was detected during fermentation,free amino acids increased significantly and extensive hydrolysis of the sarcoplasmic proteins and myfibrillar proteins occurred during storge.The proteolysis of inoculate groups was significantly more than control group,the groups of Pediococcus acidilactici and Lactobacillus-B5407 were the most.