Goose bone mud was used as raw material in order to provide substrate for preparing hot reaction meat flavor.Hydrolysate enriched amino acid was prepared through directional hydrolysis after pretreatment.Effects of pretreatment temperature and time on goose bone mud enzymatic hydrolysis were studied in the article.Enzymatic hydrolysis Conditions were optimized through orthogonal test.Free amino acids of goose bone mud hydrolysate were determined.The results showed that the best pretreatment temperature and time of goose bone mud were 80℃,40min.The best enzymatic hydrolysis condition was: trypsin 10 000 IU/g,temperature 45℃,pH 7.5,ratio of the material and extraction liquid 1∶ 30,5h.In the best enzymatic hydrolysis,degree of hydrolysis of goose bone mud was 63.09%,average peptide chain length 1.59%,Nitrogen rate of recovery 56.78%.Goose bone mud hydrolysate contained 16 free amino acids,7 essential amino acids,content of essential amino acids was 61.35%.