The changes and correlation of physiological activities,γ-aminobutyric acid(GABA) and other essential substances in germinating soybean in germinating soybean were investigated in this study.It is indicated from the result that with the increase of culture temperature and time,the shoot growth and respiration rate of the germinating soybean are enhanced,the contents of soluble sugar decrease and the content of reducing sugar,soluble protein,free amino acids and GABA increased.The content of dry matter presented the downward trend along with culture time.Correlation analysis revealed,on the one hand,that the GABA accumulation has a significant positive correlation(P<0.01) with sprout length(r=0.888),respiratory rate(r=0.917),soluble protein(r=0.878),free amino acid(r=0.943),on the other hand,has a negative correlation with content of dry matter(r=–0.915).By comprehensive consideration of quality and GABA accumulation of germinated soybean,the suitable germinating condition for high GABA germinated soybean was dark culture for 5 d at 30 °C.