Using ultrasonic extraction,O-nornuciferin,N-nornuciferin,nuciferine and roemerine in Nelumbo nucifera leaves were extracted and determined by the established HPLC method.This work provided a new method for quality control of Nelumbo nucifera leaves and its products.Four alkaloids were separated on a reversed-phase Welch Materials C18 column with a gradient elution using acetonitrile-1% triethylamine solution as mobile phase.Linear regression of O-nornuciferin,N-nornuciferin,nuciferine and roemerin in the concentration range of 0.1~50 μg/mL(R>0.999) was satisfied and the detection limit were 1.87 ng,2.22ng,2.34ng and1.88 ng(S/N=3∶ 1),respectively.The method was very precious with good recovery.It can be used for determination of alkaloids from Nelumbo nucifera leaves.