The aim of this study was to investigate dynamics of yeasts during spontaneous fermentation of wine.Yeasts associated with Cabernet Sauvignon grapes were isolated and identified.123 yeast strains were obtained,among those,71 and 52 strained were obtained in 2009 and 2010 respectively.10 WL-culture-types were obtained through colony morphology on WL medium.5 types were both isolated in the two vintages.Yeasts were differentiated to 9 types using 5.8S rDNA-ITS region RFLP analysis.And 8 specises(belonging to 7 genuses) were obtained: Aureobasidium pullulans,Hanseniaspora uvarum,Rhodotorula mucilaginosa,Pichia caribbica,Hanseniaspora vineae,Cryptococcus flavescenss,Candida zemplinina and Saccharomyce cerevisiae.Yeasts belonging to Saccharomyce genus were the main microbe during the late-mid stages of spontaneous fermentation,while in the early stages,non-Saccharomyce yeasts were dominant.