Three strains of Lactobacillus plantarum(KLDS6.0330,KLDS6.0333,KLDS6.0335) showing higher Cholesterol removal ability and bile salt hydrolase activity had screened,we determined its cholesterol assimilation,bile salt deconjugation and cholesterol co-precipitating properties.Three strains of Lactobacillus plantarum can assimilate more choleserol(﹥43μg/mL);All strains exhibited deconjugation of both sodium glycocholate and sodium taurocholate,More cholic acid was liberated from the deconjugation of sodium glycocholate than sodium taurocholate;Co-precipitation of cholesterol with cholic acid was observed from deconjugation of both sodium glycocholate and sodium taurocholate,and by all strains studied.More cholesterol was precipitated with cholic acid when sodium glycocholate was used compared to sodium taurocholate.Increased cholesterol co-precipitation with deconjugated bile was observed with decreasing pH levels,small amount of cholesterol co-precipitated with sodium glycocholate and sodium taurocholate.The results indicated that these three strains of Lactobacillus plantarum could remove cholesterol in vitro via three mechanisms.