The proper microbial strain for preparing rapeseed peptide from rapeseed meal by solid-state fermentation was obtained by means of plate cultivation and mixed fermentation,and the effects of mixed fermentation on glucosinolates degradation could be evaluated by rate of glucosinolates degradation.The results showed that the mixed strain of Bacillus subtilis and Actinomucor elegans has the best effect of mixed fermentation,and the content of low molecular weight rapeseed peptides which are produced after mixed fermentation is higher than those of the other two mixed strains.It is more suitable for producing rapeseed peptide through mixed fermentation.The mixed strain of Bacillus subtilis and Aspergillus usamii has better effect of mixed fermentation on glucosinolates degradation than those of other mixed strains,and it can be studied on detoxification in the follow-up of the experiment.