Carotenoids production through fermentation possesses great advantages.We got a strain of Sporidiobolus pararoseus which produce more than ten kinds of carotenoids.The technologies of TLC,HPLC and LC-MS were used to separation and identification of these carotenoids in the extract of fermentation products after purification by silica gel column.With the information of ultraviolet absorption spectroscopy,mass spectrum and the regular yeast metabolism pathway,four kinds of carotenoids were identified.β-carotene was one of the products of Sporidiobolus pararoseus,and the other three components could be γ-carotene,torulene,and torularhodin.