The development of non-dairy probiotic food was a vatial trend in probiotic foods.Comparing with dairy-based probiotic foods,oats-based probiotic foods can provide more benefits including hight edible fiber content,low lipid and non-lactose.The feasibility of Lactobacillus helveticus growing on the full oat substrate was studied in this paper.The influence of the pretreatment and concentration of the substrate on the growth of probitic was researched.The result showed that the growth characteristics of different lactic acid bacterias in the oat-based substrate was distinctive.Oat hydrolyzed by malts could improve the growth of Lac.helveticus.As the concentration of the substrate was 7%(W/V),the pretreated substrate added 1% malts at 60℃ for 30 min was appropriate for the growth of Lac.helveticus.The cell in this medium at 37℃was cultured for 4h to reach the stabilization period of growth.The viable cell counts after fermentation for 10h were about 108 CFU/mL.As the culture was kept in at 4℃ for 21d,the viable cell counts remained above 107 CFU/mL.