High-temperature peanut meal were used as raw materials.Colloid mill,microfluidization and enzyme pretreatment were compared on the effect of extracting protein yield from high temperature denatured peanut meal.The peanut protein isolate were analyzed by protein yield,protein purity,NSI,and its structure and functional properties.The results showed that jet cooking had 37.56% of protein yield and 82.5% of NSI;solubility and emulsification also improved significantly.After jet cooking,peanut protein isolate was completely denatured,soluble aggregation were produced and particle size became bigger;the hydrophobicity surface increased obviously.