This study was about the evaluation of a double layer plate method to detect Vibrio parahaemolyticus(V.p) in seafood.Throughout adding a certain amount of V.p in mixed cultures and seafood samples and applying frozen treatments to make V.p injured,four methods(DLAP plate,MPN method,BCVM plate and TCBS plate) were adopted to detect V.p in every experimental sample,the results of which were analyzed by SPSS software(P<0.05).The results demonstrated that the four methods were not significantly different from each other in different samples under the ideal experimental conditions.But if V.p was inactive or injured,the results of DLAP plate for detecting V.p,which could reduce at least 5 day detecting period compared to MPN method,were as effective as MPN and more accurate than BCVM plate or TCBS plate.Because of its time-saving and exactness,the DLAP plate offers a broad application for quick detecting and screening of V.p in seafood.