Methods related to the modification of cell permeability were studied systematically in Actinomucor elegans.The correlation between cell membrane permeability determined by electrical conductivity method and cell enzymatic activity for ATP production from adenine was investigated.As a result,drying method was selected.After optimization by drying method in production process,10.13 g/L ATP was accumulated from 3 g/L adenine and the molar conversion ratio was 82.76%,which increased by 202.39% compared with the control group.Electrical conductivity of treatment group was obviously higher than that of the control group and the correlation between electrical conductivity and drying time was alike with the correlation between ATP production and drying time,which proved that drying method increased cell membrane permeability and ATP production.