The growth curves,specific growth rates and productivities of Chlorella vulgaris were analyzed under autotrophic,heterotrophic and mixotrophic cultivation,as well as the changes of pH values and glucose consumption in medium,the contents and productivities of photosynthetic pigment,protein and lipid were investigated.The results indicated that the specific growth rate of C.vulgaris under mixotrophic was to 4.25~4.43times than autotrophic and 0.78~1.00 times than heterotrophic,the productivity of C.vulgaris in mixotrophic was to 5.79~6.27 times than autotrophic,and 1.11~1.31 times than heterotrophic cultivation.The values of C.vulgaris productivities for photosynthetic pigment(3.62 mg/ L.d),protein(53.41 mg/ L.d) and lipid(44.65 mg/ L.d) were obtained under mixotrophic cultivation in per liters culture liquid,all kinds of value more than autotrophic and heterotrophic.The present paper showed that the mixotrophy be more fit for the physical environments of the microalgae growth conditions,and be suited to microalgae cultivation with high density and high content of bioactive substances.