An effective method for determining glutamine in wheat oligo - peptide products with pre - column derivatization by BTI and AQC subsequently was developed.Great linear relationship existed(r =0.999 9) at the glutamine concentration range of 2～100μmol/L and the detection limits was 2μmol/L,relative standard deviation (RSD) of peak area for all standards concentration in 3 duplicates was below 1%.Recovery of standard dipeptide AQ (Ala-Gin ) was(95.94±0.22 )%.Glutamine content in wheat oligo - peptide powder was 23.54±0.49%under the optimum condition.The glutamine content in wheat oligo-peptide powder was much greater than other food-derived oligo-peptide products.Besides of the high glutamine content,the advantage of high water solubility,strong stability, strong digestivity and absorptivity,high protein content and balanced amino acid components of glutamine in wheat oligo - peptide powder will provide its widely usage in the futrue.