Food and Fermentation Industries >

2011 , Vol. 37 >Issue 12: 25 - 28

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2011.12.020

Food and Fermentation Industries

Improving the Lipase from Penicillium expansum by Error Prone PCR

  • Shi Bi-hong ,
  • Li Qiang ,
  • Chen Ming ,
  • Wu Wei-bin ,
  • Shi Qiao-qin ,
  • Wu Song-gang
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Online published: 2011-12-25

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Abstract

A random mutated lipase gene library was constructed by error prone PCR technique.4000 strains of GS115 containing random mutant lipases were primary screened.300 strains were secondary screened based on lipase activity,thermostability and acid resistance,one mutant strain named ep3 was finally selected by flask fermentation.The optimum pH and optimum working temperature of ep3 lipase was pH 9.4 and 35℃,respectively,which was the same as that of the wild type recombinant lipase(PEL-GS),and the specific activity of the lipase at pH 9.4 and 35℃ was 3 440 U/mg,which was 17% higher than that of the wild type lipase.

Key words： error prone PCR; improving; Penicillium expansum; lipase

Cite this article

Shi Bi-hong , Li Qiang , Chen Ming , Wu Wei-bin , Shi Qiao-qin , Wu Song-gang . Improving the Lipase from Penicillium expansum by Error Prone PCR[J]. Food and Fermentation Industries, 2011 , 37(12) : 25 -28 . DOI: 10.13995/j.cnki.11-1802/ts.2011.12.020

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