The dominant strain(GenBank accession number: JN248616) was isolated from the soil in Qapqal County,Xinjiang.Its growth characteristics were consistent with Clostridium botulinum of type A which belonged to thick gram-positive bacillus.Colonies in EYA culture medium showed serrated edge and irregular rough surface,with large opaque rings.Based on morphology of the strain,characteristics of physical and biochemistry,and combined with gel electrophoresis,16S rDNA sequences and phylogenetic tree,the strain was identified as Clostridium botulinum of type A.