The adsorptive thermodynamics and dynamics characteristics of Gynostemma polysaccharide on macroporous resin was studied.UV-visible spectrophotometry was used to detect the adsorptive equilibrium curve and adsorptive dynamic curve at different temperatures.The results showed that the adsorptive balance data were correlated well to Langmuir equation,second-order kinetic equation,Boyd film diffusion equation and kannan-sundaram equation.The relativities among them were all good.This adsorptive procedure was a non-spontaneous endothermic process of increased entropy.The rate of adsorption was mainly controlled by the intra-particle diffusion.