With yield and purity of crude polysaccharide as evaluation indexes,the optimal extraction process conditions of polysaccharides from Coreopsis basalis flowers by ultrasoound-assisted compound enzymolysis was studied.Effect of enzymatic hydrolysis and ultrasonic treatment on the structure of Coreopsis basalis flowers polysaccharides was evaluated through the near infrared spectral analysis,no enzyme and ultrasound-assisted treatment was used as control.The results showed that the optimal extraction process conditions were: compound enzyme 2.5%(cellulase 2%,acid protease 0.5%),temperature 55℃,pH 4.5,2.0 h.Under these conditions,the yield of crude polysaccharide was up to 9.83%,purity was 28.58%,and the purity was up to 64.57% after polyamide column purification.The result of near infrared spectral analysis showed that enzymatic hydrolysis and ultrasonic assisted extraction technology had no significant effect on the structure of Coreopsis basalis polysaccharides,but they can improve the extraction yield of polysaccharides.