To investigate the adsorption characteristics and purification process of the flavonoids from Kalimeris indica by macroreticular resin.The static capacity absorption,static capacity desorption,static adsorption dynamics(quasi-order kinetic equation of Lagergren),static adsorption isothermal(Langmuir and Freundich),dynamic absorption ratio and dynamic elution ratio of seven types of macroreticular resin were studied and compared in order to find the best one,and the adsorption and desorption performances of the best one were studied in detail.The type of D101 resin gave the best effect of separation with the sampling rate 3 BV /h and concentrations of 9.36 mg/mL in pH 4-5,and the concentration of elution was 75 % ethanol with eluting velocity 3 BV/h.The purity of total flavonoids in the product was up to 70%,and the purification process was feasible.