Food and Fermentation Industries >

2012 , Vol. 38 >Issue 01: 156 - 160

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2012.01.044

Food and Fermentation Industries

Determination of Available Lysine in Milk-Based Infant Formula Powders with 2,4,6-Trinitrobenzene Sulfonic Acid

  • Li Jun-yan ,
  • Qiu Kai ,
  • Zhong Qi-ding ,
  • Yu Xiao-jin ,
  • Ding Fu-qiang ,
  • Xiong Zheng-he ,
  • Zhang Ze-sheng
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Online published: 2012-01-25

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Abstract

A method of determination of available lysine in infant formula powder with 2,4,6-trinitrobenzene sulfonic acid(TNBS) was established.The ether usage,hydrolysis time and incubation time with TNBS were studied.Good linearity was obtained in the range of 0.02~0.12 mg/mL(r2=0.999 3);the determination limit was 3.72 μg/mL;and quantitative limit was 10.23 μg/mL and the sample spiked recoveries were in range of 86%~115% with the reproducibility RSD of 1.55%~5.57%.

Key words： infant formula; 2; 4; 6-trinitrobenzene sulfonic acid(TNBS); available lysine

Cite this article

Li Jun-yan , Qiu Kai , Zhong Qi-ding , Yu Xiao-jin , Ding Fu-qiang , Xiong Zheng-he , Zhang Ze-sheng . Determination of Available Lysine in Milk-Based Infant Formula Powders with 2,4,6-Trinitrobenzene Sulfonic Acid[J]. Food and Fermentation Industries, 2012 , 38(01) : 156 -160 . DOI: 10.13995/j.cnki.11-1802/ts.2012.01.044

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