Food and Fermentation Industries >

2012 , Vol. 38 >Issue 02: 174 - 179

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2012.02.010

Food and Fermentation Industries

Identification of Malting Barley Using the RAPD-capillary Vesselchip Electrophoresis

  • Wu Ya-jun ,
  • Wang Bin ,
  • Han Jian-xun ,
  • Zhang Zhen-min ,
  • Yang Hai-rong ,
  • Chen Ying
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Online published: 2012-02-25

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Abstract

In China malting barley is mainly dependent on import.It is necessary to establish fast and accurate method for cultivar identification to protect the benefit of brewery industry.In this paper RAPD and 2100 capillary vessel chip electrophoresis technique was applied to differentiate malting barley cultivars which were mainly used in brewery.Data of 11 repeated assays was statistically analyzed.The marker bands were selected according to reproducibility and specificity.Parameters of length and quantity of marker band were set.Seven marker bands produced by five random primers divided 19 malting barleys into 13 groups.

Key words： brewer's barley; RAPD; capillary chip electrophoresis; strain identification

Cite this article

Wu Ya-jun , Wang Bin , Han Jian-xun , Zhang Zhen-min , Yang Hai-rong , Chen Ying . Identification of Malting Barley Using the RAPD-capillary Vesselchip Electrophoresis[J]. Food and Fermentation Industries, 2012 , 38(02) : 174 -179 . DOI: 10.13995/j.cnki.11-1802/ts.2012.02.010

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