Eleven types of macroporous resins were applied to study static adsorption and desorption performances towards Trigonella foenum-graecum L.seeds flavonoids,and DM130 was the selected for the separation and purification of the crude flavonoids from Trigonella foenum-graecum L.prepared with ultrasonic-assisted extraction.Single factor experiments showed that the optimum purification procedure of total flavonoids from Trigonella foenum-graecum L.were determined as follows: sample loading amount 3.64 mg total flavonoids /g macroporous adsorption resin at pH 5.0,adsorption duration 2 h,flow rate 2.0 mL/min,70% ethanol,desorption volume 3BV.Under the above optimized purification process,the recovery of flavonoids was 85.05% and the purity increased from 7.8% to 26.5%.