The influences of different nitrogen sources on exopolysaccharide production were studied.Among them(NH4)2SO4 is the best candidate to increase the production of exopolysaccharide.Along with the rising amount of(NH4)2SO4,the molecular weight of exopolysaccharide is declining.The maximum production was 6.89g/L with 1.5 g/L(NH4)2SO4.In batch fermentation,pH and carbon source are controlled.The production of exopolysaccharide can reach 16.2 g/L in 72 h.The composition of exopolysaccharide is mainly glucose and galactose.Their proportion is about 1∶2.The polysaccharides contribute to cell wall were consisted of glucose,galactose and mannose,and their proportion is 2∶1∶1.This paper will form the foundation for the study on the physiological function of exopolysaccharide produced by yeast and the derivatization of insoluble polysaccharide.