An extraction method was developed to separate the complex reagent 1-decanol in enzymatic reaction system for α-cyclodextrin production.Firstly,a certain amount of 1-decanol was added to α-cyclodextrin solution to form α-cyclodextrin-1-decanol complexes for the parameter study of extraction separation.Effects of type and volume of extraction solvent,extraction time,and extraction temperature on extraction separation of 1-decanol were investigated.The results showed that the optimum conditions were that ethyl ether,15 times as many as the volume of 1-decanol,was used as extraction solvent to extract 1-decanol at 30℃for 30 min.Secondly,under above optimum conditions,1-decanol in enzymatic reaction system for α-cyclodextrin production was extracted.The results showed that,after extraction separation of 1-decanol,α,β and γ-cyclodextrin yields determined were almost the same as those by the traditional separation method——steam distillation,which further verified that 1-decanol in the α-cyclodextrin-1-decanol complexes could be effectively separated using extraction method.Furthermore,compared with steam distillation,the proposed extraction method was simple,energy-saving and efficient.