The research on kinetics and metabolism of esterification catalyzed by Monascus esterifying enzymes were carried out with hexanoic acid and ethanol as substrates.The results showed that the consumption of hexanoic acid subsequently grew steadily after the initial stage from 0~8h in which it increased rapidly along with the reaction time,and then reached a balance after 24 h.Inhibition was noted by excessive ethanol,but relieved partially when concentration of hexanoic acid increased.Among 15.81~142.31 mmol/L of hexanoic acid concentration,single substrate reaction was identified by positive linear relation between initial velocity and concentration of hexanoic acid.The crude Monascus esterifying preparation contained various enzymes so that the empirical model could only be established according to experiment results,which could better express the kinetics characteristic of the reaction catalyzed by the crude enzyme.Ethyl hexanoate was the main product,followed by ethyl caprylate,ethyl lionleate,ethyl oleate,ethyl palmitate and phenylacetaldehyde.The results of OAV analysis indicated that esterification catalyzed by crude Monascus esterifying preparation was appropriate for improving the quality of Chinese liquor and other products.