Extraction of protein from grape skins was investigated with reversed micelles extraction by using cationic surfactant CTAB.Effect of some parameters,such as pH value in aqueous phase,the content of surfactant,ionic strength,concentration of grape skins protein,the species and volume of organic solvent and co-solvent in reversed micellar solution as well as temperature,on the extraction ratio of grape skins protein was examined.Quadratic orthogonal rotation combination experiments were conducted to investigate the effects of concentrations of surfactant,ionic strength and pH value on the extraction rate.The forward-extraction yield of protein was found as high as 56.54% by 32.68mmol/L CTAB /Chloroform/ n-butane(4∶1,v/v)from the supernatant with pH 6.4,0.4mol/L NaCl.The best extraction condition was pH value 6.4,concentrations of surfactant 32.68mmol/L,protein concentration 1mg/mL,NaCl concentration 0.4mol/L,temperature 30℃.Under this condition: the backward extraction yield was 71.29% by 0.8 mol/L NaCl solution,temperature 20℃with pH3.8 as backwards extraction condition.