rDNA sequence-based analysis has been widely used in microbial identification.This study verified the accuracy of the rDNA sequences data deposited in GenBank.Forty-three 16S rDNA sequences of Bacillus horikoshii,twenty-two ITS(internal transcribed spacer) sequences of Aspergillus foetidus and twenty-three 26S rDNA D1/D2 region sequences of Candida boidinii were obtained from GenBank database.The result showed that out of these 88 named sequences,5 were misidentified or unreliable,17 were incomplete and too short to align,58 were unpublished and 62 were unvouchered in public collections.This study suggests that the GenBank database provided limited reference value in the microbial rDNA homology.The accuracy of related sequences needs to be verified by BLAST analysis.