The antifungal mechanism of trans-cinnamaldehyde was studied using the research pattern consisting of wild-type Saccharomyces cerevisiae strain WHU2a and three cell wall synthase-deficient strains δfks1,CHS020 and CHS003 which were derivatives of WHU2a.The results showed trans-cinnamaldehyde had an obvious inhibition effect on glucan synthase-deficient strain δfks1,the minimum inhibitory concentration(MIC) of trans-cinnamaldehyde was 8 μg/mL,which was 8 folds lower than that against the wild-type strain.Besides,the MIC against chitin synthase-deficient strains CHS020 and CHS003 were 4 folds lower as well.By growth rescue assay,it was proved that the growth inhibition effect of trans-cinnamaldehyde on δfks1 could partly be rescued by sorbitol,which confirmed the susceptibility of trans-cinnamaldehyde on cell wall.Furthermore,the contents of β-glucan and chitin on δfks1 cell wall were proved decreasing by 23.48% and 31.94%,respectively,after treated by trans-cinnamaldehyde.Based on the data presented,it is reasonable to conclude that the main antifungal mechanism of trans-cinnamaldehyde is to specifically inhibit the synthesis of β-glucan and chitin on fungal cell wall.