The wastewater of Dioscorea zingiberensis C.H.Wright using the traditional hydrolyze process technology is a kind of organism waste that is high in concentration and acidity.In this study,pretreated with the wastewater was pre-treated by Dioscorea zingiberensis C.H.Wright in three different ways,and the goal of producing Glutathione by fermentation meanwhile reducing the COD value of wastewater was achieved.The result indicated that the three different pretreatment methods influenced Glutathione yield.Given the definition of Glutathione yield ratio fermented in pure sugar liquid 100%,process 1: by only adjusting pH,the fermentation yield of Glutatione reached 0.847 625 g/L,accounting for 54.79 % of the pure sugar fermentation medium,and the removal rate of COD was 57.17 %;process 2: by adjusting pH first and then absorbed with activated carbon,the fermentation yield of Glutathione reached 0.835 7 g/L,accounting for 55.02 % of the pure sugar fermentation medium,and removal rate of COD was 77.60 %;process 3: absorbed with activated carbon first and then adjusted pH,the fermentation yield of Glutathione reached 0.689 0 g/L,accounting for 44.55 % of the pure sugar fermentation medium and removal rate of COD was 73.52 %.Taking production cost in consideration,process 2 is the best.This study has achieved good environmental and economical benefits and provided theoretical support for industrial production by recycling the valuable resources of wastewater.At present report on such study is not available at home and abroad.