The extractions of microwave enhancement extraction of polysaccharide from fresh Pleurotus eryngii were optimized by orthogonal regression design.On the basis of single-factor experiments and the principles of Box-Behnken central composite experimental design,the quadric regression orthognal optimize experiment with 4 factors and 3 Levels were used,the quadric regression linear analysis methodology were used to explore the effect of each factor on extraction rate of polysaccharide.The results shows that the optimal extraction processing conditions were: temperature 76℃,microwave time 12 min,material-water ratio 1∶35(g∶mL),microwave power 700 W and extract twice.Under the optimal extraction conditions,the extraction rate of polysaccharide from Pleurotus eryngii's fruit-body was up to 11.80%.