Food and Fermentation Industries >

2012 , Vol. 38 >Issue 03: 23 - 27

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2012.03.013

Food and Fermentation Industries

Enhancement of ε-PL production with Supplement of Auxiliary Energy Source Citric Acid by Streptomyces sp.M-Z18

  • Ren Xi-dong ,
  • Chen Xu-sheng ,
  • Dong Nan ,
  • Li Shu ,
  • Li Feng ,
  • Zhao Fu-lin ,
  • Tang Lei ,
  • Mao Zhong-gui
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Online published: 2012-03-25

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Abstract

The synthesis of ε-PL was performed by adding citric acid as auxiliary energy material.The results showed that contribution of citric acid to the fermentation of ε-PL was obvious.The optimum dose,addition time of citric acid and batch fermentations at various pH values were studied.Under the optimized condition,pH 3.8 and 20 g/L citric acid,the production of ε-PL reached 9.50 g/L and the productivity of ε-PL was 4.40 g/(L·d),it was enhanced by 46.4% and 48.1%,respectively,in contrast with original batch fermentation strategy.Moreover,the fed-batch production of ε-PL reached 22.89 g/L,2.41-fold of that in batch fermentation.

Key words： ε-poly-L-lysine; Streptomyces sp.M-Z18; citric acid; fed-batch fermentation

Cite this article

Ren Xi-dong , Chen Xu-sheng , Dong Nan , Li Shu , Li Feng , Zhao Fu-lin , Tang Lei , Mao Zhong-gui . Enhancement of ε-PL production with Supplement of Auxiliary Energy Source Citric Acid by Streptomyces sp.M-Z18[J]. Food and Fermentation Industries, 2012 , 38(03) : 23 -27 . DOI: 10.13995/j.cnki.11-1802/ts.2012.03.013

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