Food and Fermentation Industries >

2012 , Vol. 38 >Issue 03: 109 - 114

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2012.03.016

Food and Fermentation Industries

Selection of One Rhizopus sp.Mutant Strain High Producing Thermostable Lipase

  • Sun Chun-yan ,
  • Zhao Xiang-ying ,
  • Dong Xue-qian ,
  • Qiao Jun ,
  • Liu Jian-jun
Expand

Online published: 2012-03-25

Fold

Abstract

A high enzyme activity mutant strain UD-23 was selected from a Thermostable Lipase-producing Rhizopus SFE-L01 which was stored in our laboratory by using UV,DES and UV + DES mutagenetic treatments.Compare to the original strain,the mutant's enzyme activity increased by 1.12 times and reached 24 000.00 U/mL.Study of the production conditions and enzymatic characteristics of the lipase from UD-23 showed that the optimal temperature of the lipase from UD-23 was 60℃.After the optimization,the enzyme activity of this mutant strain reached 30 000.00U/mL,increased by 25%.

Key words： thermostable lipase; Rhizopus; mutation breeding

Cite this article

Sun Chun-yan , Zhao Xiang-ying , Dong Xue-qian , Qiao Jun , Liu Jian-jun . Selection of One Rhizopus sp.Mutant Strain High Producing Thermostable Lipase[J]. Food and Fermentation Industries, 2012 , 38(03) : 109 -114 . DOI: 10.13995/j.cnki.11-1802/ts.2012.03.016

Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.