In order to optimize the fermentation process to direct the production of physiologically active substances,with the mycelial biomass as a reference index,the composition of culture medium were optimized.The study determined that the mycelial culture was the most suitable carbon source,and nitrogen source,trace element and optimization various additions of medium components was obtained: glucose was 40.0 g/L,peptone was 3.5g/L,KH₂PO_4 was 0.5 g/L,MgSO_4·7H₂O was 3.5 g/L,and CaCl_2 was 0.3 g/L.The biomass of mycelia was studied through Box-Benhnken design and response surface methodology(RSM),which enabled effective examination on the parameters with a moderate number of experiments.The results showed that the biomass of mycelia was well correlated with operating parameters,including rotate speed,inoculation number and time,with a determination coefficient R~2 of 0.9838 and small P value.The improved response surface model is helpful in the calculation of the biomass of mycelia,and makes quality and efficient mycelia pellet culturing possible.