In this study,the influence of the decarboxylase(EC 4.1.1.72) gene ARO10 cloning and overexpression on the metabolic flux analysis of 3-(methylthio)-1-propanol biosynthesis in Saccharomyces cerevisiae INVSc1 was investigated.Results showed that the introduction of the recombinant plasmid pYES2-ARO10 containing ARO10 gene into S.cerevisiae INVSc1 by using the lithium acetate method resulted in the overexpression of decarboxylase.And the level of 3-(methylthio)-1-propanol biosynthesis in the recombinant strain SC10-1 was significantly enhanced.The maximum yield of 0.90 g/L was achieved when the recombinant strain was incubated for 120 h.Compared with the control strain,the yield increased by 55.2%.These results clearly indicated the decarboxylase from S.cerevisiae s288c is a key rate-limiting enzyme in the biosynthesis of 3-(methylthio)-1-propanol,and the overexpression of ARO10 gene is helpful to the 3-(methylthio)-1-propanol biosynthesis.