To provide an effective means for species identification and epidemiological tracing for Cronobacter spp.strains by studying molecular characteristic of reference strains and strains isolated from samples,38 Cronobacter spp.strains were identified by genotyping test-Genomic DNA was extracted by methods of Randomly Amplified Polymorphic DNA analysis(RAPD).One effective primer was selected with which each strain could be amplified 1 to 7 DNA fragments of 400~2 000bp.8 reference strains were identified into separate species groups.Similar to 50% for the standard,38 strains could be classified into 8 genotyping groups by genetic differences.RAPD method in this study can be applied to species identification and epidemiological tracing for Cronobacter spp.strains.