In this paper,Saccharomyces cerevisiae cell wall proteins were taken for the application of whole cell extracted from cell wall proteins,dilute alkaline buffer extracted proteins from purified cell wall and Lysozyme and β-glucanase enzyme solution hydrolysis of purified cell wall to extracted cell wall proteins all this three extraction methods,at the same time.The differences between different algebra yeast cell wall proteins has been identified and analyzed.The results show that:the method of lysozyme and β-glucanase enzyme solution hydrolysis of purified cell wall to extract cell wall proteins can obtain more cell wall protein bands and higher purity,therefore this method is determinted the best extraction method for yeast cell wall protein extraction.Withal,by SDS-PAGE electrophoresis analysis found different generation of yeast cell wall proteins with a large difference and identified molecular weight of 36 kDa,17 kDa and 12 kDa are the three main different cell wall proteins for different generation yeasts.Among that,the expression of 36 kDa,17 kDa protein bands decreased with the age of yeast increasing,whereas,12 kDa protein band expression increased with the age of yeast.